Coding

Part:BBa_K2926011

Designed by: Ina Schmitt   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-15)


Cas13a sgRNA array with 3 sgRNAs (for A. niger)

This part codes for a Cas13a guideRNA cluster with three guide RNAs targeting essential genes in A. niger.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 38
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 38
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 38
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 38
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None